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If this email is no longer relevant please= update your communication preferences <a href=3D"https://www.selectscience= .net/SSUserAcc.aspx?mailID=3D13540&email=3Ddr.sultan@jack.ngo" style=3D= "color: #888; text-decoration: underline;" target=3D"_blank">here</a>.</spa= n><br>You can also read this as a <a href=3D"https://www.selectscience.net/= commNWDetails.aspx?mailID=3D13540&email=3Ddr.sultan@jack.ngo" style=3D"= color: #888; text-decoration: underline;" target=3D"_blank">web version</a>= .<img border=3D"0" height=3D"1" src=3D"https://www.selectscience.net/trackO= penRate.aspx?mailID=3D13540&email=3Ddr.sultan@jack.ngo" width=3D"1"></p= ></td></tr><tr style=3D"background-color: inherit;"><td height=3D"20" style= =3D"font-size: 1px;"> </td></tr><tr><td width=3D"660" style=3D"text-al= ign: center;"><table cellpadding=3D"0" cellspacing=3D"0" align=3D"center" w= idth=3D"600"><tbody><tr><td width=3D"298" style=3D"text-align: left;"><a hr= ef=3D"https://www.selectscience.net/go/?itemID=3D8322&linkid=3Dheaderte= xt&itemTypeID=3D4&mailID=3D13540&email=3Ddr.sultan@jack.ngo&= ;url=3Dhttps%3A%2F%2Fwww.selectscience.net%2Fregister.aspx%3FitemID%3D53263= %26itemTypeID%3D10%26clickID%3D3%26utm_source%3DMembers%26utm_medium%3DExcl= usive-Email%26utm_campaign%3DHorizonDecember2020" style=3D"text-decoration:= underline; color: #10638d; font-size: 18px;" target=3D"_blank">Download Ap= plication Note >></a></td><td width=3D"4" style=3D"font-size: 1px;"= > </td><td width=3D"298" style=3D"text-align: right;"><a href=3D"https= ://www.selectscience.net/go/?itemID=3D8322&linkid=3Dlogo&itemTypeID= =3D4&mailID=3D13540&email=3Ddr.sultan@jack.ngo&url=3Dhttps%3A%2= F%2Fhorizondiscovery.com%2F" style=3D"text-decoration: none; color: inherit= ;" target=3D"_blank"><img alt=3D"horizon - Insipired Cell Solutions" src=3D= "https://selectscience.net/images/mailshots/horizon/horizon-logo-1220.png" = width=3D"260" height=3D"65" border=3D"0" style=3D"border-color: transparent= ; border: 0;"></a></td></tr></tbody></table></td></tr><tr style=3D"backgrou= nd-color: inherit;"><td height=3D"18" style=3D"font-size: 1px;"> </td>= </tr><tr><td width=3D"660" style=3D"text-align: center;"><table cellpadding= =3D"0" cellspacing=3D"0" align=3D"center" width=3D"600"><tbody><tr><td widt= h=3D"600" style=3D"text-align: center;"><strong style=3D"font-size: 20px; c= olor: #000;">Off-target effects: disturbing the silence of RNA interference= (RNAi)</strong></td></tr></tbody></table></td></tr><tr style=3D"background= -color: inherit;"><td height=3D"15" style=3D"font-size: 1px;"> </td></= tr><tr><td width=3D"660" style=3D"text-align: center;"> <a href=3D"https= ://www.selectscience.net/go/?itemID=3D8322&linkid=3Dbanner&itemType= ID=3D4&mailID=3D13540&email=3Ddr.sultan@jack.ngo&url=3Dhttps%3A= %2F%2Fhorizondiscovery.com%2Fen%2Fgene-modulation%2Fknockdown%2Fsirna" targ= et=3D"_blank"><img alt=3D" " src=3D"https://selectscience.net/images/mailsh= ots/horizon/ex102320202.png" width=3D"660" height=3D"179" border=3D"0" styl= e=3D"border-color: transparent; border: 0;"> </a></td></tr><tr style=3D"bac= kground-color: inherit;"><td height=3D"18" style=3D"font-size: 1px;"> = </td></tr> <tr> <td> = <table align=3D"center" border=3D"0" cellp= adding=3D"0" cellspacing=3D"0" class=3D"email100px" width=3D"660px" style= =3D"padding: 0px 0px 0px 0px; background-color: #fff;"> = <tbody> = <tr> <td align=3D"cente= r" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" class=3D"email100px" wi= dth=3D"660px" style=3D"padding: 10px 0px 0px 0px; background-color: #fff;">= <table align=3D"cen= ter" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" class=3D"email100px" = width=3D"600px" style=3D"padding: 0px 0px 0px 0px; background-color: #fff;"= > <tbody> = <tr> = = <td align=3D"left" class=3D"email= 100px" style=3D"width: 615px; padding-top: 10px; padding-bottom: 0px; paddi= ng-right: 0px; padding-left: 0px; color: #000; background-color: #ffffff; f= ont-family: calibri, Arial, helvetica, sans-serif; font-size: 15px; text-a= lign: left; line-height: 1.2;" valign=3D"top" width=3D"615"> = = <table align=3D"left" border= =3D"0" cellpadding=3D"0" cellspacing=3D"0" class=3D"email100px" width=3D"30= 5"> = <tbody> = = <tr> = <td align=3D"center" class=3D"email100px" style= =3D"line-height: 12pt; width: 310px; padding-top: 5px; padding-bottom: 0px;= padding-right: 5px; padding-left: 5px; color: #000; background-color: #fff= fff; font-family: calibri, Arial, helvetica, sans-serif; font-size: 18px; = text-align: left; line-height: 1.2;" valign=3D"top" width=3D"310">Off-targe= t effects occur when an siRNA is processed by the RNA-Induced Silencing Com= plex (RISC) and down-regulates unintended targets. As these changes in gene= expression can lead to measurable phenotypes (such as false positives) it = is of great importance to understand the mechanism behind off-targeting so = that strategies can be developed to minimize their effects. We have discove= red that bioinformatics, novel chemical modifications, and siRNA pooling si= gnificantly decrease off-target effects.<br /><br /> = These three = strategies are incorporated into the Dharmacon™ ON-TARGETplus™ = and SMARTpool™ siRNA reagents. = <br /> = <br />&nbs= p; = = </td> = </tr> = = </tbody> = </table> = <table align=3D"right" border=3D"= 0" cellpadding=3D"0" cellspacing=3D"0" class=3D"email100px" width=3D"290"> = <= tbody> = = <tr> = = <td align=3D"center" class=3D"email100px" = style=3D"width: 290px; padding-top: 15px; padding-bottom: 0px; padding-righ= t: 0px; padding-left: 0px; color: #000; background-color: #ffffff; font-fam= ily: calibri, Arial, helvetica, sans-serif; font-size: 15px; text-align: c= enter; line-height: 1.2;" valign=3D"top" width=3D"290"><a href=3D"https://w= ww.selectscience.net/go/?itemID=3D8322&linkid=3Dimg101&itemTypeID= =3D4&mailID=3D13540&email=3Ddr.sultan@jack.ngo&url=3Dhttps%3A%2= F%2Fwww.selectscience.net%2Fregister.aspx%3FitemID%3D53263%26itemTypeID%3D1= 0%26clickID%3D3%26utm_source%3DMembers%26utm_medium%3DExclusive-Email%26utm= _campaign%3DHorizonDecember2020" style=3D"color: #303030; 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color: inherit;" t= arget=3D"_blank"><img alt=3D" " src=3D"https://selectscience.net/images/mai= lshots/horizon/ex102320204.jpg" width=3D"280" height=3D"173" border=3D"1" s= tyle=3D"border-color: black; border: 1;"></a></td><td width=3D"40" style=3D= "font-size: 1px;"> </td><td width=3D"280" style=3D"text-align: center;= "><table cellpadding=3D"0" cellspacing=3D"0" align=3D"center" width=3D"280"= ><tbody><tr><td width=3D"280" style=3D"text-align: left; font-size: 18px;">= Stock up and save 20% on all 10 nmol Smartpool siRNA</td></tr><tr style= =3D"background-color: inherit;"><td height=3D"30" style=3D"font-size: 1px;"= > </td></tr><tr><td width=3D"280" style=3D"text-align: left;"><table w= idth=3D"250" align=3D"left" style=3D"width: 250px; background-color: #D0E2E= 7;" border=3D"0" cellspacing=3D"0" cellpadding=3D"0"><tbody><tr><td height= =3D"50" align=3D"center" style=3D"color: #D0E2E7; font-size: 20px;"><a styl= e=3D"color: #D0E2E7; line-height: 50px; text-decoration: none; display: blo= ck;" href=3D"https://www.selectscience.net/go/?itemID=3D8322&linkid=3Dd= iscountcta&itemTypeID=3D4&mailID=3D13540&email=3Ddr.sultan@jack= .ngo&url=3Dhttps%3A%2F%2Fhorizondiscovery.com%2Fen%2Fgene-modulation%2F= knockdown%2Fsirna%2Fproducts%2Fon-targetplus-sirna-reagents-human%23descrip= tion" target=3D"_blank"><span style=3D"font-size: 20px;"><span style=3D"col= or: #10638D;"><strong style=3D"text-decoration: underline;">Receive discoun= t</strong></span></span></a></td></tr></tbody></table></td></tr></tbody></t= able></td></tr></tbody></table></td></tr><tr style=3D"background-color: inh= erit;"><td height=3D"40" style=3D"font-size: 1px;"> </td></tr><tr styl= e=3D"background-color: #e7e7e6;"><td align=3D"center" class=3D"email100px" = style=3D"padding: 0px; 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