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} .auto-style2 { height: 46px;= } .auto-style3 { height: 23px; }</style></head><body class= =3D"em_body" style=3D"margin:0px auto; padding:0px;" bgcolor=3D"#fafafa"><p= style=3D"font-size: 10px; color: #888; font-family: Arial, Helvetica, sans= -serif; text-align: center;" align=3D"center"> Your latest product and appl= ication news from <a style=3D"color: #888;" href=3D"http://www.selectscienc= e.net/" target=3D"_blank">SelectScience®</a>.<br />You are subscribed t= o this service. If this email is no longer relevant, please update your com= munication preferences <a style=3D"color: #888;" href=3D"http://www.selects= cience.net/SSUserAcc.aspx?mailID=3D13091&email=3Ddr.sultan@jack.ngo" target= =3D"_blank" >here</a>.<br />You can also read this as a <a style=3D"color: = #888;" href=3D"http://www.selectscience.net/commNWDetails.aspx?mailID=3D130= 91&email=3Ddr.sultan@jack.ngo" target=3D"_blank" >web version</a>. <img= border=3D"0" src=3D"http://www.selectscience.net/trackOpenRate.aspx?mailID= =3D13091&email=3Ddr.sultan@jack.ngo" width=3D"1" height=3D"1" /></p><!-= - =3D=3D Header Section =3D=3D --><!-- =3D=3D //Header Section =3D=3D --><!= -- =3D=3D Body Section =3D=3D --><table bgcolor=3D"#fafafa" border=3D"0" ce= llpadding=3D"0" cellspacing=3D"0" class=3D"em_full_wrap" width=3D"100%"><tb= ody><tr><td align=3D"left"></td><td align=3D"center" valign=3D"top"><table = align=3D"center" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" class=3D"= em_main_table" style=3D"width:600px; table-layout:fixed; background-color:#= FFFFFF;" width=3D"600"><!-- Banner section --><tbody><tr style=3D""><tr><td= align=3D"left" class=3D"auto-style1" pardot-region=3D"text4" style=3D"font= -size:12px; line-height:18px; font-family:'Helvetica', Arial, sans-serif; c= olor:#222222;"><u><a href=3D"https://www.selectscience.net/go/?itemID=3D786= 9&linkID=3Dheadercta&itemTypeID=3D4&mailID=3D13091&email=3D= dr.sultan@jack.ngo&URL=3Dhttp%3A%2F%2Fgo.fortebio.com%2Fl%2F83942%2F202= 0-07-03%2Fbknj7l%3Fcmp%3D7014u000001x4A3" target=3D"_blank">Download app no= te overview >></a></u> </td></tr><td align=3D"center" pardot-region=3D"b= anner" valign=3D"top"><a href=3D"https://www.selectscience.net/go/?itemID= =3D7869&linkID=3Dheader&itemTypeID=3D4&mailID=3D13091&email= =3Ddr.sultan@jack.ngo&URL=3Dhttps%3A%2F%2Fwww.fortebio.com%2F" target= =3D"_blank"><img src=3Dhttps://www.selectscience.net/images/mailshots/forte= bio/FB_IMG_Logo_Sartorius_Brand_450px.jpg></a></td></tr><!-- //Banner secti= on --><!-- Headline option section --><!-- //Headline option section --><!-= - Headline option1 section --><tr pardot-repeatable=3D"headline option1" st= yle=3D""><td align=3D"center" valign=3D"top"><table align=3D"center" border= =3D"0" cellpadding=3D"0" cellspacing=3D"0" class=3D"em_wrapper" style=3D"wi= dth:600px; background-color:#008CA8;" width=3D"600"><tbody><tr><td align=3D= "center" style=3D"padding:18px;" valign=3D"top"><table align=3D"center" bor= der=3D"0" cellpadding=3D"0" cellspacing=3D"0" class=3D"em_wrapper" style=3D= "width:564px;" width=3D"564"><tbody><tr style=3D""><td align=3D"center" cla= ss=3D"em_yellow" pardot-region=3D"text2" style=3D"font-size:20px; line-heig= ht:22px; font-family:'Helvetica', Arial, sans-serif; color:#FFA500; font-we= ight:bold;" valign=3D"top"><span style=3D"color:#FFFFFF;">Compare BLI, SPR,= & ITC in Affinity Characterization</span></td></tr></tbody></table></t= d></tr></tbody></table></td></tr><!-- //Headline option1 section --><!-- im= age section --><tr pardot-repeatable=3D"banner section" style=3D""><td alig= n=3D"center" valign=3D"top"><table align=3D"center" border=3D"0" cellpaddin= g=3D"0" cellspacing=3D"0" class=3D"em_wrapper" style=3D"width:600px;" width= =3D"600"><tbody><tr style=3D""><td align=3D"center" pardot-region=3D"banner= 1" valign=3D"top" class=3D""><a href=3D"http://go.fortebio.com/l/83942/2020= -07-03/bknj7l?cmp=3D7014u000001x4A3" style=3D"text-decoration:none;" target= =3D"_blank"><img src=3Dhttps://www.selectscience.net/images/mailshots/forte= bio/scientist_600px2.jpg> </a></td></tr></tbody></table></td></tr><!-- //im= age section --><!-- Headline option2 section --><!-- //Headline option2 sec= tion --><!-- full width text section --><tr pardot-repeatable=3D"full width= text without button section" style=3D""><td align=3D"center" class=3D"em_p= ad" style=3D"padding:20px 18px 0px;" valign=3D"top"><table align=3D"center"= border=3D"0" cellpadding=3D"0" cellspacing=3D"0" class=3D"em_wrapper" styl= e=3D"width:564px;" width=3D"564"><tbody><tr style=3D""><td align=3D"left" c= lass=3D"em_black em_center" pardot-region=3D"text4" style=3D"font-size:12px= ; line-height:18px; font-family:'Helvetica', Arial, sans-serif; color:#2222= 22;">Label-free surface-based analytical methods such as Bio-Layer Interfer= ometry (BLI) and Surface Plasmon Resonance (SPR) together with Isothermal C= alorimetry (ITC) and ELISA are widely used for affinity characterization of= biomolecular interaction studies and for the derivation of Affinity consta= nts (KD).<br><br>The BLI platform offers an advanced fluidics-free approach= with a wide variety of off-the-shelf biosensors for rapid binding kinetics= and quantitation analysis. It can be used for both binding characterizatio= n and screening of different types of biologics due to its better ease of u= se configurations and low maintenance attributes. While SPR and ITC are als= o used to monitor binding interactions, BLI technology offers advantages th= at include:<ul><li>Low volume and crude sample analysis</li><li>Minimal ana= lyst interaction which reduces the potential for errors that may lead to hi= gh data variability</li><li>Real-time detection mode speeding up assay trou= bleshooting for rapid optimization</li><li>Options for design of experiment= s through which various assay conditions can be evaluated simultaneously</l= i></ul>Our <strong><a href=3D"https://www.selectscience.net/go/?itemID=3D78= 69&linkID=3Dtextcta&itemTypeID=3D4&mailID=3D13091&email=3Dd= r.sultan@jack.ngo&URL=3Dhttp%3A%2F%2Fgo.fortebio.com%2Fl%2F83942%2F2020= -07-03%2Fbknj7l%3Fcmp%3D7014u000001x4A3" style=3D"text-decoration:none;colo= r:#008CA8;" target=3D"_blank">application overview</a></strong> discusses t= he accurate determination of affinity constants with real examples from lit= erature comparing data from the three technologies.</td></tr><tr><td class= =3D"em_h20" height=3D"30" style=3D"height:30px; font-size:0px; line-height:= 0px;"> </td></tr></tbody></table></td></tr><!-- //full width text sect= ion --><!--cta section--><tr pardot-repeatable=3D"cta section" style=3D""><= td align=3D"center" class=3D"em_aside15" style=3D"padding:0px 18px;" valign= =3D"top"><table align=3D"center" border=3D"0" cellpadding=3D"0" cellspacing= =3D"0" class=3D"em_wrapper" style=3D"width:564px;" width=3D"564"><tbody><tr= ><td align=3D"center" valign=3D"top"><table align=3D"center" border=3D"0" c= ellpadding=3D"0" cellspacing=3D"0" style=3D"width:246px; border-radius:3px;= background-color:#008CA8;" width=3D"246"><tbody><tr><td align=3D"center" c= lass=3D"em_blue3" height=3D"46" pardot-region=3D"btn1" style=3D"font-size:1= 5px; font-family:Arial, sans-serif; color:#0066FF;"><a href=3D"https://www.= selectscience.net/go/?itemID=3D7869&linkID=3Ddownloadbutton&itemTyp= eID=3D4&mailID=3D13091&email=3Ddr.sultan@jack.ngo&URL=3Dhttp%3A= %2F%2Fgo.fortebio.com%2Fl%2F83942%2F2020-07-03%2Fbknj7l%3Fcmp%3D7014u000001= x4A3" style=3D" display:block; line-height:46px;text-decoration:none;color= :#0066FF;" target=3D"_blank"><span style=3D"font-size:16px;"><span style=3D= "font-family:arial,helvetica,sans-serif;"><span style=3D"color:#FFFFFF;">Do= wnload Application Overview</span></span></span></a></td></tr></tbody></tab= le></td></tr><tr><td class=3D"em_h20" height=3D"20" style=3D"height:20px; f= ont-size:0px; line-height:0px;"> </td></tr></tbody></table></td></tr><= !-- //cta section--><!-- two coloum text section--><!-- //two coloum text s= ection--><!-- two coloum btn section--><!-- //two coloum btn section--><!--= image and text1 section--><!-- //image and text1 section--><!-- image and = text2 section--><!-- //image and text2 section--><!-- image and text3 secti= on--><!-- //image and text3 section--><!-- image and text4 section--><!-- /= /image and text4 section--><!-- image and text5 section--><!-- //image and = text5 section--><!-- image and text6 section--><!-- //image and text6 secti= on--><!-- full widthimage and text section--><!-- //full widthimage and tex= t section--><!--CTA Section--><!--CTA Section--><!-- two coloum image secti= on--><!-- //two coloum image section--><!-- Full width text section --><!--= //Full width text section --><!--Separate CTA section--><!-- //Separate CT= A section--></tbody></table></td></tr></tbody></table><!-- =3D=3D //Body Se= ction =3D=3D --><!-- =3D=3D Footer Section =3D=3D --><table bgcolor=3D"#faf= afa" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" class=3D"em_full_wrap= " width=3D"100%"><tbody><tr><td align=3D"center" valign=3D"top"><table alig= n=3D"center" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" class=3D"em_m= ain_table" style=3D"width:600px; 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