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Return-Path: <bounces+13288428-34de-dr.sultan=jack.ngo@em2869.selectscience.com> Delivered-To: dr.sultan@jack.ngo Received: from mocha3025.mochahost.com by mocha3025.mochahost.com with LMTP id SEWhFy01DF+P5C0AaMJ/gg (envelope-from <bounces+13288428-34de-dr.sultan=jack.ngo@em2869.selectscience.com>) for <dr.sultan@jack.ngo>; Mon, 13 Jul 2020 06:19:25 -0400 Return-path: <bounces+13288428-34de-dr.sultan=jack.ngo@em2869.selectscience.com> Envelope-to: dr.sultan@jack.ngo Delivery-date: Mon, 13 Jul 2020 06:19:25 -0400 Received: from o1.ptr5290.selectscience.com ([167.89.92.117]:10016) by mocha3025.mochahost.com with esmtps (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384:256) (Exim 4.92) (envelope-from <bounces+13288428-34de-dr.sultan=jack.ngo@em2869.selectscience.com>) id 1juvYT-00CdvG-5R for dr.sultan@jack.ngo; Mon, 13 Jul 2020 06:19:25 -0400 DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=selectscience.com; h=mime-version:from:to:subject:content-type:content-transfer-encoding; s=sgd; bh=65dFXh6HRFXDCFSxoAvNM1R4N18btFWpFoO2YtV2U34=; b=HJdGbr R5ToLJ9t/8t9aRkKBtzNglU8FhfiI5NR0CF68nWQMs/AbllO3ukxO7DY2w9UYOIE 4rlwv3fMBMY0U6koZSD12ku7ClkYmKBANq1ZcERBrbQku+5KAF2MvY9sXO2Z0Jam s7TyEeggsS6WUnRw9qFqDjx1w8IVvdjQjB53s= Received: by filter1665p1mdw1.sendgrid.net with SMTP id filter1665p1mdw1-1098-5F0C3500-5 2020-07-13 10:18:40.40775018 +0000 UTC m=+45669.274251241 Received: from id15374 (unknown) by ismtpd0004p1lon1.sendgrid.net (SG) with ESMTP id E-sOkxYlSf6Mxc8iS_J1sA for <product-news@selectscience.com>; Mon, 13 Jul 2020 10:18:40.041 +0000 (UTC) MIME-Version: 1.0 From: "SelectScience" <product-news@selectscience.com> To: dr.sultan@jack.ngo Date: Mon, 13 Jul 2020 10:18:44 +0000 (UTC) Subject: Expert article: Top tips for experimental success with KO cell lines Content-Type: text/html; charset=utf-8 Content-Transfer-Encoding: quoted-printable Message-ID: <E-sOkxYlSf6Mxc8iS_J1sA@ismtpd0004p1lon1.sendgrid.net> X-SG-EID: UwtGQ0rc3CbRjQC0x1TuLuSGvUMhLWSExK+Nj+wrLxzBwW0iyWsAD4X3CDS3Es5rvOtM4iI0UduIOm Sse6PAL4MffyT0F1T3IJu3W5uKvgHR9zlT0arn80uMUjNS5S7HzxzWg+ta8WkPCMMxbcJh4ZTMkwVd wWLZN6YQykMYHRteYG8lzQAnMjlLc1CYi4ikCPUoqenUaidIoFzwctLLFHqHSeb1LKgDdcOe6kE3oB Y= <!DOCTYPE html><html lang=3D"en" xmlns=3D"http://www.w3.org/1999/xhtml"><ti= tle></title><head> <meta charset=3D"utf-8" /> <title></title></head><= style> html, body { margin: 0px; padding: 0px; font= -family: Calibri, Arial, Helvetica, sans-serif; background-colo= r: #e7e7e6; } table { border: none; } img { borde= r: none; } @media screen and (max-width: 480px){ *[class= =3Dtddissapear] { width: 0px !important; heig= ht: 0px !important; display: none !important; } = *[class=3Demail100px-paddingbottom] { padding-bot= tom: 35px !important; } } </style><body leftmargin=3D"0" right= margin=3D"0" topmargin=3D"0" marginheight=3D"0" offset=3D"0" align=3D"cente= r"> <p style=3D"font-size: 10px; color: #888; font-family: Arial, Helvetica= , sans-serif; text-align: center;" align=3D"center"> Your latest product an= d application news from <a style=3D"color: #888;" href=3D"http://www.select= science.net/" target=3D"_blank">SelectScience®</a>.<br />You are subscr= ibed to this service. 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height: auto;"> <tabl= e cellpadding=3D"0" cellspacing=3D"0" align=3D"center" width=3D"660" border= =3D"0" style=3D"background-color: #fff; = height: auto;"> <tbody> = <tr> <td = style=3D"background-color: #fff; width: 660px; padding-top: 15px;" valign= =3D"top" width=3D"660"> = <img src=3D"https://www.selec= tscience.net/images/mailshots/abcam/May_2020/banner.jpg" alt=3D"Abcam Banne= r" width=3D"660" height=3D"288"/> = </td> </tr> = </tbody> </table> = </td> </tr> = <tr> <td align=3D"ce= nter" width=3D"660px" style=3D"width: 660px; background-color: #fff;"> = <table cellpadding=3D"0" cellspacing=3D"0" align= =3D"center" width=3D"660" border=3D"0" style=3D"background-color: #fff;"> = <tbody> = <tr> <td> = <table cellpadding=3D"0" cellspacing=3D"0" a= lign=3D"center" width=3D"615" border=3D"0" style=3D"background-color: #fff;= "> <tbody> = <tr> = <td align=3D"center" style=3D"line-height:= 20pt; text-align: center; background-color: #fff; width: 575px; padding: 1= 5px 20px 10px 20px; font-size: 18pt; color: #000; font-family: calibri, Ari= al, helvetica, sans-serif;" valign=3D"top" width=3D"575"> <a hre= f=3D"https://www.selectscience.net/go/?itemID=3D10049&linkID=3Darticlet= itle&itemTypeID=3D4&mailID=3D12808&email=3Ddr.sultan@jack.ngo&a= mp;url=3Dhttps%3A%2F%2Fwww.selectscience.net%2Fproduct-news%2Fknow-your-tar= get-how-to-achieve-research-success-with-knockout-models%3Fartid%3D51850%26= utm_source%3DMembers%26utm_medium%3DExclusive-Email%26utm_campaign%3DAbcam-= July-2020" style=3D"text-decoration: none; color: #000;" target=3D"_blank">= <strong>Key considerations for working with KO cell lines and thei= r essential role in reproducible research</strong> </a> = </td> = </tr> </tbody> = </table> = </td> </tr> = </tbody> = </table> </td> </tr> = <tr> = <td align=3D"center" width=3D"660px" style=3D"width: 660px; backg= round-color: #fff;"> <table align=3D"center"= width=3D"660" border=3D"0" cellspacing=3D"0" cellpadding=3D"0" style=3D"ba= ckground-color: #fff;"> <tbody> = <tr> = <td> <table align=3D"cen= ter" width=3D"615" border=3D"0" cellspacing=3D"0" cellpadding=3D"0" style= =3D"padding: 20px 0px 15px 0px; background-color: #fff;"> = <tbody> = <tr> = <td> = <table align=3D"left" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" = width=3D"310"> = <tbody> = = <tr> = <td align=3D"left" style=3D"line-= height: 12pt; width: 295px; padding-top: 30px; padding-bottom: 10px; paddin= g-right: 10px; padding-left: 5px; color: #222222; background-color: #ffffff= ; font-family: calibri, Arial, helvetica, sans-serif; font-size: 14pt; tex= t-align: left; line-height: 1.2;" valign=3D"top" width=3D"295"> = Dr Ha= nna Dreja, Abcam=E2=80=99s Quality Control Scientist, offers <strong>top ti= ps</strong> for planning knockout experiments and details how her team is <= strong>working with CRISPR to improve validation</strong> and speed researc= h pipelines. =C2=A0 = = </td> = </tr> = <tr> = <td align=3D"cen= ter" style=3D"width: 230px; padding-top: 0px; padding-bottom: 0px; paddin= g-right: 40px; padding-left: 40px; color: #ffffff; background-color: #fffff= f; font-family: calibri, Arial, helvetica, sans-serif; font-size: 15px; te= xt-align: center; line-height: 1.4" valign=3D"top" width=3D"230"> = = <table align=3D"center" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" = width=3D"160px"> = <tbody> = <tr> = = <td style=3D"background-color: #ffffff; font-family: calibri,= Arial, helvetica, sans-serif; font-size: 14px; color: #ffffff; width: 160= px; font-weight: normal; text-align: center; padding-top: 4px" width=3D"160= px"> = <table align=3D"right" border=3D"0" cellpad= ding=3D"0" cellspacing=3D"0" width=3D"160px"> = = <tbody> = <tr> = = <td style=3D"background-color: #ad18ac; font-family: calibr= i, Arial, helvetica, sans-serif; font-size: 16pt; width: 160px; color: #fff= fff; font-weight: normal; text-align: center; padding: 5px;"><a href=3D"htt= ps://www.selectscience.net/go/?itemID=3D10049&linkID=3Darticlecta&i= temTypeID=3D4&mailID=3D12808&email=3Ddr.sultan@jack.ngo&url=3Dh= ttps%3A%2F%2Fwww.selectscience.net%2Fproduct-news%2Fknow-your-target-how-to= -achieve-research-success-with-knockout-models%3Fartid%3D51850%26utm_source= %3DMembers%26utm_medium%3DExclusive-Email%26utm_campaign%3DAbcam-July-2020"= style=3D"color: #ffffff; text-decoration: none" target=3D"_blank">Read Int= erview</a></td> = </tr> = = </tbody> = </table> = = </td> = </tr> = </tbody> = </t= able> = </td> = </tr> = </tbody> = </table> = <td> = <table align=3D"right" border=3D"0" cellpadding=3D= "0" cellspacing=3D"0" width=3D"305"> = <tbody> = = <tr> = <td align=3D= "center" style=3D"width: 305px; padding-top: 0px; padding-bottom: 10px; pa= dding-right: 0px; padding-left: 0px; color: #222222; background-color: #fff= fff; font-family: calibri, Arial, helvetica, sans-serif; font-size: 15px; = text-align: center; line-height: 1.2;" valign=3D"top" width=3D"305"> = = <a href=3D"https://www.selectscience.net/go/?itemID=3D10049&linkID= =3Darticleimg&itemTypeID=3D4&mailID=3D12808&email=3Ddr.sultan@j= ack.ngo&url=3Dhttps%3A%2F%2Fwww.selectscience.net%2Fproduct-news%2Fknow= -your-target-how-to-achieve-research-success-with-knockout-models%3Fartid%3= D51850%26utm_source%3DMembers%26utm_medium%3DExclusive-Email%26utm_campaign= %3DAbcam-July-2020" style=3D"color: #ffffff; text-decoration: none" target= =3D"_blank"><img alt=3D"Dr Hanna Dreja" height=3D"152" src=3D"https://www.s= electscience.net/images/mailshots/abcam/hannadrejav2.jpg" style=3D"height: = 152px; width: 152px;" width=3D"152"/></a></td> = </tr> = = </tbody> = </table> = = </td> = </td> = </tr> = </tbody> </table> = </td> = </tr> </tbody> = </table> </td> = </tr> <tr> <td align=3D"= center" width=3D"660" style=3D"background-color: #fff; height: auto;"> = <table cellpadding=3D"0" cellspacing=3D"0" align= =3D"center" width=3D"660" border=3D"0" style=3D"background-color: #fff; = height: auto;"> = <tbody> <tr> = <td align=3D"center" style=3D"padding-top: 5p= x; text-align: center; background-color: #fff; width: 660px;" width=3D"660"= > <table align=3D"center" st= yle=3D"text-align: center; width: 595px" width=3D"595px"> = <tbody> = <tr> = <td width=3D"595px" style=3D"border-top: 1px solid #4474c5; widt= h: 595px; text-align: center" align=3D"center"> = = </td> = </tr> </t= body> </table> = = </td> </tr> = </tbody> </table> = </td> </tr> = <tr> <td align=3D"cente= r" width=3D"660px" style=3D"padding: 10px 0px 40px 0px; width: 660px; backg= round-color: #fff;"> <table align=3D"center"= width=3D"660" border=3D"0" cellspacing=3D"0" cellpadding=3D"0" style=3D"ba= ckground-color: #fff;"> <tbody> = <tr>= <td> = <table align=3D"center" width=3D"615" border=3D"0" cel= lspacing=3D"0" cellpadding=3D"0" style=3D"background-color: #fff;"> = <tbody> = = <tr> = <td> <table= align=3D"left" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"= 310"> = <tbody> = = <tr> = <td align=3D"left" style=3D"width: 300px; p= adding-top: 10px; padding-bottom: 5px; padding-right: 0px; padding-left: 10= px; color: #222222; background-color: #ffffff; font-family: calibri, Arial,= helvetica, sans-serif; font-size: 14pt; text-align: left; line-height: 1.= 2;" valign=3D"top" width=3D"300"> = <span style=3D"font-size: 18pt; = color: #95a3a4"><strong>Knockout cell lines <br /> </strong></span> = = = <table align=3D"center" width=3D"300" border=3D"0" ce= llspacing=3D"0" cellpadding=3D"0" style=3D"background-color: #fff;"> = <tbody> = = <tr> = <td align= =3D"center" width=3D"30px" style=3D"padding: 0px 0px 3px 0px; vertical-alig= n: top; font-size: 15pt; width: 30px; background-color: #fff;">• = </td> = <td align= =3D"left" width=3D"270px" = style=3D"vertical-align: top; background-color: #fff; padding= : 0px 0px 3px 0px; width: 270px; color: #000; = font-size: 14pt; line-height: 17pt; font-= family: calibri, arial, helvetica, sans-serif;"> = Engineered and ready for you. Reliable, off-= the-shelf, single-gene knockouts = </td> = </tr> = <tr> = <td align=3D"center" = width=3D"30px" style=3D"vertical-align: top; font-size: 15pt; width: 30px; = background-color: #fff;">• </td> = = <td align=3D"left" width=3D"270px" = style=3D"vertical-align= : top; background-color: #fff; padding: 0px 0px 3px 0px; width: 270px; colo= r: #000; fon= t-size: 14pt; line-height: 17pt; font-family: calibri, arial, helvetica, sa= ns-serif;"> Lar= ge and expanding range of knockouts in various=C2=A0 immortalized mammalian= cell lines</td> </t= r> = = </tbody> </table> = = </td> = </tr> = <tr> = <td align=3D"center" style=3D"width: 310px; = padding-top: 0px; padding-bottom: 15px; padding-right: 0px; padding-left: 0= px; color: #ffffff; background-color: #ffffff; font-family: calibri, Arial,= helvetica, sans-serif; font-size: 15px; text-align: center; line-height: = 1.4" valign=3D"top" width=3D"310"> = <table align=3D"center" borde= r=3D"0" cellpadding=3D"0" cellspacing=3D"0" width=3D"220px"> = = <tbody> = <tr> = <td style=3D"backg= round-color: #ffffff; font-family: calibri, Arial, helvetica, sans-serif; = font-size: 14px; color: #ffffff; width: 220px; font-weight: normal; text-al= ign: center; padding-top: 4px" width=3D"220px"> = = <table align=3D"center" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" w= idth=3D"220px"> = <tbody> = = <tr> = <td style=3D"bo= rder: 1px #2f528f; background-color: #4472c4; line-height: 17pt; font-fami= ly: calibri, Arial, helvetica, sans-serif; font-size: 15pt; width: 220px; c= olor: #ffffff; font-weight: normal; text-align: center; padding: 6px;"><a h= ref=3D"https://www.selectscience.net/go/?itemID=3D10049&linkID=3Dcta2&a= mp;itemTypeID=3D4&mailID=3D12808&email=3Ddr.sultan@jack.ngo&url= =3Dhttps%3A%2F%2Fwww.abcam.com%2Freagents%2Fknockout-cell-lines%3Futm_sourc= e%3DSelectScience%26utm_medium%3Dpaid_referral%26utm_campaign%3DGene_engine= ering" style=3D"color: #ffffff; text-decoration: none" target=3D"_blank">Fi= nd your KO cell line</a></td> = </tr> = = </tbody> = </table> = = </td> = </tr> = </tbody> = = </table> = </td> = </tr> = </tbody> = </table> = <td> = <table align=3D"right" border=3D"0" c= ellpadding=3D"0" cellspacing=3D"0" width=3D"305"> = <tbody> = = <tr> = = <td align=3D"center" style=3D"width: 295px; padding-top: 10px; padding-bo= ttom: 0px; padding-right: 0px; padding-left: 10px; color: #222222; backgrou= nd-color: #ffffff; font-family: calibri, Arial, helvetica, sans-serif; fon= t-size: 14pt; text-align: left; line-height: 1.2;" valign=3D"top" width=3D"= 305"> = = <span style=3D"font-size: 18pt; color: #95a3a4"><s= trong>Knockout cell lysates <br /> </strong></span> = = = <table align=3D"center" width=3D"300" bor= der=3D"0" cellspacing=3D"0" cellpadding=3D"0" style=3D"background-color: #f= ff;"> <tbody> = = <tr> = = <td align=3D"center" width=3D"30px" style=3D"padding: 0px 0px 3px 0px; ver= tical-align: top; font-size: 15pt; width: 30px; background-color: #fff;">&b= ull; </td> = = <td align=3D"left" width=3D"270px" = style=3D"vertical-align: top; background-color: #ff= f; padding: 0px 0px 3px 0px; width: 270px; color: #000; = font-size: 14pt; line-height: 1= 7pt; font-family: calibri, arial, helvetica, sans-serif;"> = Edited and ready to use = </td> = </tr> = <tr> = = <td align=3D"center" width=3D"30px" style=3D"vertical-align: top; fo= nt-size: 15pt; width: 30px; background-color: #fff;">• &nbs= p; </td> = <td align=3D"left"= width=3D"270px" = style=3D"vertical-align: top; background-color: #fff; padding: 0px 0px= 3px 0px; width: 270px; color: #000; = font-size: 14pt; line-height: 17pt; font-family: c= alibri, arial, helvetica, sans-serif;">Lysate range immediate access in a s= tandardized format</td> = </tr> <tr> = = <td align=3D"center" width=3D"30p= x" style=3D"vertical-align: top; font-size: 15pt; width: 30px; background-c= olor: #fff;">• </td> = = <td align=3D"left" class=3D"email100px-paddingbottom" widt= h=3D"270px" = style=3D"vertical-align: top; background-color: #fff; padding: 0px 0px 25px= 0px; width: 270px; color: #000; = font-size: 14pt; line-height: 17pt; font-family: calib= ri, arial, helvetica, sans-serif;"> = Ready to go.<br /> = </td> = </tr> = </tbody> = </table> = = </td> = </tr> = <tr> = <td align= =3D"center" style=3D"width: 305px; padding-top: 0px; padding-bottom: 15px;= padding-right: 0px; padding-left: 0px; color: #ffffff; background-color: #= ffffff; font-family: calibri, Arial, helvetica, sans-serif; font-size: 15p= x; text-align: center; line-height: 1.4" valign=3D"top" width=3D"305"> = = <table align=3D"center" border=3D"0" cellpadding=3D"0" cellspacing= =3D"0" width=3D"220px"> = <tbody> = <tr> = = <td style=3D"background-color: #ffffff; font-family: c= alibri, Arial, helvetica, sans-serif; font-size: 14px; color: #ffffff; wid= th: 220px; font-weight: normal; text-align: center; padding-top: 4px" width= =3D"220px"> = <table align=3D"center" border=3D"0"= cellpadding=3D"0" cellspacing=3D"0" width=3D"220px"> = = <tbody> = <tr> = = <td style=3D"background-color: #4472c4; line-height= : 17pt; font-family: calibri, Arial, helvetica, sans-serif; font-size: 15pt= ; width: 220px; color: #ffffff; font-weight: normal; text-align: center; pa= dding: 6px;"><a href=3D"https://www.selectscience.net/go/?itemID=3D10049&am= p;linkID=3Dcta3&itemTypeID=3D4&mailID=3D12808&email=3Ddr.sultan= @jack.ngo&url=3Dhttps%3A%2F%2Fwww.abcam.com%2Freagents%2Fknockout-lysat= es%3Futm_source%3DSelectScience%26utm_medium%3Dpaid_referral%26utm_campaign= %3DGene_engineering" style=3D"color: #ffffff; text-decoration: none" target= =3D"_blank">Find your lysate</a></td> = </= tr> = </tbody> = </ta= ble> = </td> = </tr> = <= /tbody> = </table> = </td> = </tr> = </tbody> = </table> = = </td> = </td> = </tr> = </tbody> </table> = </td> = </tr> </tbody> = </table> </td> = </tr> <tr> = <td width=3D"660px" align=3D"center" style=3D"pa= dding: 0px; border: 0px; width: 660px; font-= family: Arial,helvetica,sans-serif; text-align: center; color: #888888; = font-size: 10px"> = <table cellpadding=3D"0" cellspacing=3D"0" border=3D"0" align=3D"center">= <tr> = <td width=3D"600px" align=3D"center" style=3D"padding: 20px 0px 10px 0= px; border: 0px; width: 600px; f= ont-family: Arial,helvetica,sans-serif; text-align: center; color: #888888;= font-size: 10px"> = <table cellpadding=3D"0" cellspacing=3D"0" bo= rder=3D"0" align=3D"center"> = <tr> <td valign=3D"midd= le" width=3D"148px" align=3D"center" style=3D"padding: 0px; border: 0px; = width: 198px; font-fam= ily: Arial,helvetica,sans-serif; text-align: center; 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